Acta Zoologica Academiae Scientiarum Hungaricae 46 (3), pp. 181–195, 2000

Title: Developmental biology and larval morphology of Chromatomyia fuscula (Zetterstedt) (Dipt., Agromyzidae)

Authors: Darvas, B.*, A. Andersen**, A. Szappanos*** and L. Papp***

Authors' addresses: *Plant Protection Institute, Hungarian Academy of Sciences, Budapest, PO Box 102, H-1525, Hungary
** Norwegian Crop Research Institute, Plant Protection, Fellesbygget, N-1432 ás, Norway
*** Hungarian Natural History Museum, Budapest, PO Box 137, H-1431, Hungary

Abstract: The egg stadium of Chromatomyia fuscula (Zetterstedt, 1838) (collected in Southern Norway) takes 4.5 days to hatch in June. The first instar larva (4.5–6.5 days) is metapneustic and the 2nd instar (6.5–9.5 days) is amphipneustic. The third instar has a pair of fan-like anterior spiracles with 18–20 digits each and a pair of posterior spiracles with 5–8 pores, each arranged in 2 rows. Pupariation takes place between the 12.5th and 15th day. During sclerotization and melanization firstly blackish "V" shape stripes develop on the ventral puparial side which enlarge later and merge to a wide black spot, but the dorsal side remains usually yellowish and transparent. Pupal moult occurs within the puparial shell 2–3 days after the white pre-puparial phase. After 4 days, the imaginal transformation takes place. After a further 5 days, the pharate phase adult with white eyes turns to the orange eye phase. The following day, the red eye phase occurs. The next day bristle formation takes place: wings and bristles are greyish, later turn to black. During the following 1–2 days, the procuticle phase adult emerges. Adults are not attracted to either young or old spring barley plants, but look for a sheltered, humid place. A very long imaginal aestivation and hibernation period starts, which is a very uncommon overwintering strategy among agromyzid flies.

Key words: Chromatomyia fuscula, Agromyzidae, morphology, developmental stages

Acta Zoologica Academiae Scientiarum Hungaricae 46 (3), pp. 197–229, 2000

Title: Revision of Newsteadia of the Australian and Pacific regions, with description of eleven new species (Homoptera: Coccoidea, Ortheziidae)

Authors: Kozár, F. and Zs. Konczné Benedicty

Authors' address: Plant Protection Institute, Hungarian Academy of Sciences
H-1525 Budapest, Pf. 102, Hungary
E-mail: h2405koz@ella.hu

Abstract: Eleven new species of Newsteadia are described, and the already known four species are redescribed from the regions studied. A key is presented for the identification of 15 species currently included in the genus from these regions. A modified concept of the genus is presented and several new characters are given as diagnostic of the genus. The genus Transnewsteadia is synonymized. The zoogeography of the genus is discussed.

Key words: Homoptera, Coccoidea, Ortheziidae, Newsteadia, Australia, Pacific Region

Acta Zoologica Academiae Scientiarum Hungaricae 46 (3), pp. 231–238, 2000

Title: Three new species of Malcus Stål, 1859 (Heteroptera, Malcidae) from Vietnam

Author: E. Kondorosy

Author's address: Department of Zoology, University of Veszprém
Georgikon Faculty of Agricultural Sciences, H-8360 Keszthely, Hungary

Abstract: Descriptions of three new Malcus species, M. vasarhelyii, M. spinosus and M. stysi from Vietnam are given. Data of the Malcus specimens preserved in the Hungarian Natural History Museum, identified by the author, are also presented.

Key words: new species, Malcus, Malcidae, Heteroptera, Vietnam

Acta Zoologica Academiae Scientiarum Hungaricae 46 (3), pp. 239–248, 2000

Title: Two new Australian species of Crotonia (Acari: Oribatida), with new records of Crotonioidea from the Australian region

Author: Z. Olszanowski

Author's address: Department of Animal Taxonomy and Ecology, A. Mickiewicz University, Szamarzewskiego 91 A, 60–569 Poznan, Poland
E-mail: olszanow@main.amu.edu.pl

Abstract: New oribatid mite species, Crotonia ovata sp. n. and C. tryjanowskii sp. n. are described from Australia (Tasmania and New South Wales respectively) from temperate rain forest and Nothofagus moorei forest. An identification key for adults of the six Crotonia species of Australia is included. New records of some species of crotonioid mites (Crotonioidea) from the Australian region are given. The first record of Holonothrus artus Olszanowski from the eastern Hemisphere (so far only noticed in Chile) is worthy of notice.

Key words: Australian region, Acari, Oribatida, Crotonioidea, taxonomy, zoogeography

Acta Zoologica Academiae Scientiarum Hungaricae 46 (3), pp. 249–257, 2000

Title: Two new species of Drosophilidae (Diptera) from Central Europe

Author: L. Papp

Author's address: Department of Zoology, Hungarian Natural History Museum
H-1431 Budapest, Pf. 137, Hungary
E-mail: lpapp@zoo.zoo.nhmus.hu

Abstract: Leucophenga hungarica L. Papp, sp. n. and Stegana (Steganina) consimilis L. Papp et Máca, sp. n. are described from the 1999 collectings from Hungary (additional specimens are from the Czech and the Slovak Republics). With 20 original figures.

Key words: Leucophenga, Stegana (Steganina), new species, Drosophilidae, Hungary

Acta Zoologica Academiae Scientiarum Hungaricae 46 (3), pp. 259–264, 2000

Title: Establishing microsatellite analysis for locally endangered populations of root vole (Microtus oeconomus)

Authors: Papp, T.*, Gubányi, A.** and Rácz, G.***

Authors' addresses: *Veterinary Medical Research Institute, Hungarian Academy of Sciences, H-1143 Budapest, Hungária krt. 21, Hungary, E-mail: tpapp@novell.vmri.hu
** Department of Zoology, Hungarian Natural History Museum, 1088 Budapest, Baross u. 13, Hungary, E-mail: gubanyi@zoo.zoo.nhmus.hu
*** Museum of Southwestern Biology, Biology Department, The University of New Mexico Albuquerque, NM-87131, USA

Abstract: The root voles (Microtus oeconomus) is endangered in Hungary; the monitoring of its fragmented and isolated populations is necessary for an effective nature conservation management. Analysing of microsatellite DNA has been shown to be an efficient method to assess genetic variability among a wide variety of organisms. The present paper describes how this technique can be used to study populations of root voles. Two different DNA sampling and extraction methods and 10 heterologous primer pairs were tested. From these 10 primer pairs, 6 were found appropriate for microsatellite analysis of this species. Sequence analysis of the amplified PCR products raised the problem that length polymorphism may be the result of variation of a compound microsatellite locus.

Key words: Microtus oeconomus, microsatellite analysis, sequence analysis, Hungary