Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 263–276, 2017

Title: A new species of Polycelis (Platyhelminthes, Tricladida, Planariidae) from China

Authors: Zi-Mei Dong, Guang-Wen Chen*, He-Cai Zhang and De-Zeng Liu

Authors' address: College of Life Science, Henan Normal University, Jianshe Road No. 46, Xinxiang 453007, Henan, China; *E-mail:

Abstract: In this paper, a new species of Polycelis of the family Planariidae from China is described. Mature individuals have 80–140 eyespots; the testes are well-developed and most of them occupy the entire dorso-ventral space; the penis is a long cone with well-developed musculature; the boundary between the penis bulb and penis papilla is vague and the bulbar cavity is not observed; the bursal canal is surrounded by a well-developed coat of circular muscles, and a thin layer of longitudinal muscles. The karyotype shows a diploid complement of 38 chromosomes, with the formula 2n = 38 = 24m + 14sm.

Key words: Platyhelminthes, Tricladida, Polycelis, new species, China.

DOI: 10.17109/AZH.

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 277–292, 2017

Title: Redescriptions of three species of the genus Neobisium from the Balkans and the Carpathians (Arachnida: Pseudoscorpiones)

Authors: János Novák1 and Christoph Hörweg2

Authors' addresses: 1Eötvös Loránd University, Department of Systematic Zoology and Ecology, H-1117 Budapest, Pázmány Péter sétány 1/C, Hungary; E-mail:
2Natural History Museum Vienna, 3. Zoology (Invertebrates), Burgring 7, 1010 Vienna, Austria; E-mail:

Abstract: Redescriptions of Neobisium (Neobisium) polonicum Rafalski, 1937, N. (N.) bosnicum Beier, 1939 and N. (N.) bosnicum herzegovinense Beier, 1939 are given, accompanied with new illustrations of the most important morphological characters. N. (N.) bosnicum herzegovinense Beier, 1939 is elevated to species rank as Neobisium (N.) herzegovinense Beier, 1939. N. (N.) biharicum Beier, 1939 is proposed as a junior subjective synonym of N. (N.) polonicum. New records of N. (N.) polonicum from Romania are presented.

Key words: pseudoscorpions, Neobisiidae, taxonomy, synonymy, new records.

DOI: 10.17109/AZH.

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 293–307, 2017

Title: A morphometric and molecular study of the genus Pseudopodisma (Orthoptera: Acrididae)

Authors: Máté Kisfali1, Péter Sólymos2, Antal Nagy3, István A. Rácz1, Orsolya Horváth4 and Gábor Sramkó4,5

Authors' addresses: 1Department of Evolutionary Zoology and Human Biology, University of Debrecen, H-4010 Debrecen, P.O. Box 3, Hungary; E-mails:;
2Alberta Biodiversity Monitoring Institute and Department of Biological Sciences, CW 405, Biological Sciences Bldg., University of Alberta, Edmonton, Alberta, T6G 2E9, Canada E-mail:
3Institute of Plant Protection, University of Debrecen, H-4032 Debrecen, Böszörményi út 138, Hungary; E-mail:
4Department of Botany, University of Debrecen, H-4010 Debrecen, Egyetem tér 1, Hungary E-mails:;
5MTA-DE “Lendület” Evolutionary Phylogenomics Research Group, H-4010 Debrecen, Egyetem tér 1, Hungary

Abstract: The specific status of three described taxa belonging to the genus Pseudopodisma (P. fieberi, P. transilvanica, P. nagyi) is based on a single character, because the original descriptions separate the species solely on the basis of genital structure of the males, and the three species are seemingly indistinguishable based on external morphology. To test this taxonomic separation we conducted morphometric and molecular investigations in the genus. Males were a priori identified by the original descriptions of the taxa, and from 18 external morphometric characters collected, the six most eligible characters of males and females were selected for linear discriminant analysis. The linear discriminant based classification agreed for 35 (85.3%) male and 30 (88.2%) female specimens out of the 41 a priori identified males and 34 females. Besides morphometrics, we sequenced the cytochrome b gene with newly devised primers in all species plus two outgroups. The phylogenetic analysis of resulting sequences indicated a strong species-delimitation within the genus Pseudopodisma (i.e., multiple individuals of the a priori species formed monophyletic clades). Thus, taking the morphometric and molecular results into consideration, we conclude the validation of specific status of Pseudopodisma fieberi, P. nagyi and P. transilvanica within the genus.

Key words: CytB, mitochondrial primers, grasshopper systematics, linear discriminant analysis, multivariate morphology.

DOI: 10.17109/AZH.

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 309–325, 2017

Title: Scale insect (Hemiptera, Coccomorpha) survey of soil samples from southern Asia with description of two new species of Rhizoecidae

Authors: Mehmet Bora Kaydan1,2, Zsuzsanna Konczné Benedicty1, Douglas J. Williams3 and Éva Szita1

Authors' addresses: 1Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences H-1022 Budapest, Herman Ottó u. 15, Hungary; E-mail:
2Çukurova University, Imamoglu Vocational School, Adana, Turkey; E-mail:
3Department of Life Sciences (Entomology), The Natural History Museum, Cromwell Road, London SW7 5BD, UK

Abstract: Seventeen species of Ortheziidae, Rhizoecidae and Pseudococcidae (Hemiptera: Coccomorpha) were extracted from East and South Asian soil sample collection of the Hungarian Natural History Museum. Among 1080 soil samples almost 100 included scale insect specimens. Two new species of the family Rhizoecidae, Rhizoecus muranyii Kaydan sp. n. and Ripersiella danyii Kaydan et Konczné Benedicty sp. n. are described and illustrated, and identification keys of East and South Asian Rhizoecus and Ripersiella species are provided.

Key words: hypogeal scale insects, fauna, Berlese funnel apparatus.

DOI: 10.17109/AZH.63.3.309.2017

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 327–341, 2017

Title: Three new species and a new genus of Hypselostomatidae (Gastropoda: Pulmonata) from Cong Troi Cave, Northern Vietnam

Authors: Barna Páll-Gergely1, Adrienne Jochum3,4 and Takahiro Asami2

Authors' addresses: 1Department of Biology, Shinshu University, Matsumoto 390-8621, Japan; E-mail:
2Department of Biology, Shinshu University, Matsumoto 390-8621, Japan; E-mail:
3Naturhistorisches Museum der Burgergemeinde Bern, CH-3005 Bern, Switzerland; E-mail:
4Institute of Ecology and Evolution, University of Bern, CH-3012 Bern, Switzerland

Abstract: The Hypselostomatidae Zilch, 1959 comprise a family of minute pupilloid snails usually endemic to limestone habitats in single hills, adjacent hill ranges, and in caves of Southeast Asia. Except for one molecular study, their taxonomy is largely conchologically driven. In this work, shell material derived from sediment samples from the Cong Troi Cave in Northern Vietnam is examined and taxonomically assessed. Altogether, five species are recognized. Three new species are described: Angustopila stochi Páll-Gergely et Jochum, sp. n., Tonkinospira tomasini Páll-Gergely et Jochum sp. n. and Dentisphaera maxema Páll-Gergely et Jochum, sp. n. For the latter species, Dentisphaera Páll-Gergely et Jochum a new genus is erected. Another hypselostomatid species is found to be identical with the original sample of Angustopila subelevata Páll-Gergely et Hunyadi, 2015 from Guangxi, China. This is the first record of A. subelevata from Vietnam. The fifth species closely resembles Pupisoma sp. from Thailand. Though figured here, it is not assessed taxonomically in this work.

Key words: cave snails, limestone habitats, Vietnam, micro snails.

DOI: 10.17109/AZH.63.3.327.2017

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 343–354, 2017

Title: Reproductive characteristics and habitat selection of Carabus ulrichii (Coleoptera, Carabidae) in woodland habitats in Hungary

Authors: Ferenc Kádár1, Rita Andorkó2 and Zoltán Elek3

Authors' addresses: 1Centre for Agricultural Research, Plant Protection Institute, Hungarian Academy of Sciences, H-1022 Budapest, Herman Ottó út. 15, Hungary; E-mail:
2Department of Systematic Zoology and Ecology, Faculty of Sciences, Institute of Biology, Eötvös Loránd University, H-1117 Budapest, Pázmány Péter sétány 1/C, Hungary; E-mail:
3MTA-ELTE-MTM Ecology Research Group, Institute of Biology, Eötvös Loránd University, H-1117 Budapest, Pázmány Péter sétány 1/C, Hungary; E-mail:

Abstract: The distribution, seasonality and reproductive characteristics of Carabus ulrichii (Coleoptera, Carabidae) were studied in woodland habitats in Hungary. We sampled a beech forest, an oak forest and the adjacent transition zone using pitfall traps, in the Pilis Biosphere Reserve in 2005 and 2006. Generalised linear models were used to explore the impacts of the habitat quality and seasonality on the distribution of this species. We found that the light intensity model explained best the species’ activity density pattern, suggesting preference for open oak forest. We also found that the mandible wear was not a good estimator of age in C. ulrichii, but ovarial condition could be used to assess reproductive status. The seasonal activity and life history patterns were similar in the three different habitats for both sexes. Although differences in the total activity density of the species were found, there were no differences either in the seasonal activity patterns, or in the age-composition, or in the reproductive characteristics of C. ulrichii among the studied habitats. C. ulrichii beetles reproduced only once per season in the studied forested habitats.

Key words: Carabus ulrichii, forest habitats, seasonal activity, reproduction, age-composition.

DOI: 10.17109/AZH.63.3.343.2017

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 355–360, 2017

Title: Additional polymorphic microsatellite loci for detailed population genetic studies of Lethrus apterus (Coleoptera: Geotrupidae)

Authors: Rita Rácz1,2, Judit Bereczki1,2, András Kosztolányi1,3, Attila Horváth4, Szabolcs Sziráki5 and Zoltán Barta1,2

Authors' addresses: 1MTA-DE “Lendület” Behavioural Ecology Research Group, Department of Evolutionary Zoology, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary; E-mails:,,
2Department of Evolutionary Zoology, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary
3Department of Ecology, University of Veterinary Science Budapest, H-1077 Budapest, Rottenbiller utca 50, Hungary; E-mail:
4Genomic Medicine and Bioinformatic Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Egyetem tér 1, Hungary; E-mail:
5University of Debrecen, Faculty of Informatics, H-4028 Debrecen, Kassai út 26, Hungary; E-mail:

Abstract: In this study we attempted to isolate 13 additional microsatellites from the beetle Lethrus apterus and then to use them to screen for polymorphisms in 35 specimens collected from the northern Hungarian mountains. The newly developed markers presented in this paper are polymorphic, including between two and seven detected alleles per locus. The observed and expected heterozygosity per locus ranged between 0.057 and 0.686 and between 0.056 and 0.756, respectively, while two loci showed significant deviations from Hardy-Weinberg equilibrium. These deviations can be explained by the presence of null alleles. All loci cross-amplified in at least two out of four congeneric Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun). The polymorphic microsatellite markers we present for L. apterus will be valuable for future population genetic studies.

Key words: dinucleotide repeats, trinucleotide repeats, fragmentation, species conservation, parentage.

DOI: 10.17109/AZH.63.3.355.2017

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Acta Zoologica Academiae Scientiarum Hungaricae 63 (3), pp. 361–376, 2017

Title: Genetic profile analysis of aquaculture Russian sturgeon (Acipenser gueldenstaedtii) – implications for restocking measures

Authors: Alexandru Burcea, Gina-Oana Popa, Iulia Elena Florescu, Andreea Dudu, Sergiu Emil Georgescu and Marieta Costache

Authors' address: University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, Bucharest, Splaiul Independenței 91–95, Sector 5, 050095, Romania; E-mails:,,,,

Abstract: The aim of this study is to help the conservation effort of the Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833 population from Lower Danube, ensuring its sustainable development in regards to aquaculture and wild populations. We evaluated the genetic diversity of five aquaculture strains using molecular markers, determining the suitability of employing the individuals in ex situ conservation and in restocking programs. The mitochondrial cytochrome b gene analysis highlighted that some sequences from aquaculture and wild populations are grouped together representing a single haplotype. Results showed that the five aquaculture strains are grouped in 3 haplotypes with a haplotype diversity of 0.55686. The microsatellite analysis highlights that the majority of loci have a tetrasomic profile that makes the inferring of genotypes and the statistical analyses difficult, only two (AciG198 and Spl106) had a disomic profile that could prove useful in future studies. We can conclude that the molecular analysis is important for the genetic characterization of aquaculture strains, shedding light on the genetics of A. gueldenstaedtii.

Key words: aquaculture, microsatellites, cytochrome b, genetic diversity, Romania strains.

DOI: 10.17109/AZH.63.3.361.2017

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